Composite

Part:BBa_K4613311:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


pET-PC-SUMO-M-CPA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 152
    Illegal BglII site found at 474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 584
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We attached 6*his tag to the N-terminus of M-CPA to get purified M-CPA. We attached SUMO-tag to the N-terminus of M-CPA to increase the rate of solube protein expression.


Source

NAU-CHINA 2023

References